l-Fucose Synthesis Using a Halo- and Thermophilic l-Fucose Isomerase from Polyextremophilic Halothermothrix orenii

l-Fucose isomerase (l-FucI)-mediated isomerization is a promising biotechnological approach for synthesizing various rare sugars of industrial significance, including l-fucose. Extremozymes that can retain their functional conformation under extreme conditions, such as high temperature and salinity, offer favorable applications in bioprocesses accompany harsh conditions. To date, only one thermophilic l-FucI has been characterized l-fucose synthesis. Here, we report from Halothermothrix orenii (HoFucI) which exhibits both halophilic properties. When evaluated biochemical HoFucI exhibited optimal activities at 50–60 °C pH 7 with 0.5–1 M NaCl the presence 1 mM Mn2+ cofactor. The results obtained here show unique feature polyextremozyme, facilitates production using this enzyme.